Investigation of the Laxative Activity of Methanolic Extract of Tamarix Aphylla L. Karst (Saltcedar) In Experimental Animals

Authors

  • Khushal Khan MPhil Scholar, Department of Pharmacology, Institute of Basic Medical Sciences, Peshawar, Pakistan.
  • Niaz Ali Associate Professor, Department of Pharmacology, Institute of Basic Medical Sciences, Peshawar, Pakistan.
  • Nawaz Sharif MPhil Scholar, Department of Pharmacology, Institute of Basic Medical Sciences, Peshawar, Pakistan.
  • Rooh Ullah MPhil Scholar, Department of Pharmacology, Institute of Basic Medical Sciences, Peshawar, Pakistan.
  • Tauseef Ahmad MPhil Scholar, Department of Pharmacology, Institute of Basic Medical Sciences, Peshawar, Pakistan.
  • Mohsin Ali Muhammad College of Medicine, Peshawar

Keywords:

Tamarix Aphylla, Constipation, Laxative, Charcoal meal, senna glycosides

Abstract

Background: Phytomedicines present potential uses because pharmacological activities of different herbs have not been explored completely. Tamarix aphylla (L.) Karst, mentioned in the Holy Quran, is one amongst different plants used traditionally as laxative. Keeping in view the traditional use of Tamarix aphylla as laxative, current work was carried out to determine its use on scientific grounds.

Method: The methanolic extract of Tamarix Aphylla (L.) Karst was prepared. The lethal dose (LD50) was calculated. Charcoal meal gastrointestinal transit test and weight of the faeces matter were used for evaluation of the laxative potential of the extract. For charcoal meal gastrointestinal transit test, mice were divided into four groups of six animals each, the first group as a negative control, second group as a positive control (Neostigmine) while group third and fourth were treated with methanolic extract of Tamarix Aphylla at doses of 150mg/kg, 300mg/kg and 450mg/kg p.o. respectively. For the weight of the faecal matter method, rats were divided into five groups of six animals each, the first group as a negative control, second group as a positive control (Neostigmine) while group third, fourth and fifth were treated with methanolic extract of Tamarix Aphylla at doses of 150mg/kg, 300mg/kg and 450mg/kg p.o. respectively.

Results:  The series of experiments showed that the LD50 for the methanolic extract of Tamarix Aphylla in animal models was 1650mg/Kg. A significant increase in movement of the charcoal meal was produced at 300mg/kg dose of extract when compared to the normal control group (p-value <0.05). In weight of the faeces matter method, the faecal output of rats was increased significantly at doses of 300 and 450 mg/kg when compared to the normal control group (p-value <0.05).

Conclusion: The results proved that the methanolic extract of Tamarix Aphylla (L.) Karst has a significant laxative activity.

Published

2021-12-10

How to Cite

Khan, K. ., Ali, N., Sharif, N., Ullah, R., Ahmad, T., & Ali, M. (2021). Investigation of the Laxative Activity of Methanolic Extract of Tamarix Aphylla L. Karst (Saltcedar) In Experimental Animals. THE STETHO, 2(12), 1-9. Retrieved from http://thestetho.com/index.php/ts/article/view/43